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KMID : 0624620090420030154
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BMB Reports
2009 Volume.42 No. 3 p.154 ~ p.159
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The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor
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Zhou Wu
Chen Haixiang
Zhang Lihuang
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Abstract
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JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor.
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KEYWORD
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Histone demethylase, hPc2, JARID1B, Transcriptional co-repressor
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